Aldi has transitioned the majority of its lorries in Swindon to operate on hydrotreated vegetable oil fuel.
The supermarket chain has made the change to 63 of the 65 HGVs at its Swindon distribution centre, with similar numbers converted in two other regions.
The move is expected to reduce Aldi's carbon footprint by around 15,432 tonnes of CO2e each year.
Hydrotreated vegetable oil is a diesel alternative which produces lower greenhouse gas emissions than regular diesel.
If Aldi converted its entire UK fleet of more than 550 HGVs to hydrotreated vegetable oil, it could reduce its carbon footprint by an estimated 55,130 tonnes of CO2e each year.
Liz Fox, national sustainability director at Aldi, said the move is part of the company's "long-term sustainability goals".
She added: "Switching to hydrotreated vegetable oil fuels on our lorry fleet aligns with our long-term sustainability goals and reflects our commitment to reducing our carbon emissions.
"We are always looking for ways to continue our efforts to drive positive change across the business and this move in particular is a significant milestone for Aldi."
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